Luis Hernandez-Tovias
LSAMP Scholar
Major: Neuroscience
Mentors: Heather Hundley, Priyanka Mukherjee, and Ananya Mahapatra
Adenosine Deaminases Acting on RNA (ADAR) participate in RNA editing and function as RNA binding proteins however, ADAR3 only participates in RNA binding and are found to be overexpressed in Glioblastoma. A RIP-Seq assay was used to identify ADAR3 bound targets. Out of 1435 of the targets identified, about 30% are IncRNA’s. How is it that ADAR3 regulate the expression of these IncRNA’s? In order to test the effects, ADAR3 was overexpressed and compared to a cell line with regular expression. Interestingly, ADAR3 has also been found to inhibit ADAR2 RNA editing, Therefore, a cell line was created in which ADAR2 was cloned and overexpressed. Cell lines could be used to test the genes found to be regulated by ADAR3. Essentially, Long Non-Coding RNA can consist of Sense/ Antisense RNAs (AS) which are transcribed from opposite strands of DNA. These antisense genes are particularly important for epigenetic modifications such as RNA editing and Translation which both affect RNA levels of target genes. Overexpressing ADAR3 in a U373 cell line provides the possibility of identifying its role in Glioblastoma gene expression. The results determined that ADAR3 has no major impact on gene expression in sense genes; Results provide a reason to explore antisense genes, as well as protein expression.
Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. IN LSAMP is supported by NSF Award Number: HRD-1618408, 2016-2022.
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