NCGAS is teaching a course in genomics, called GEMS: WORKFLOWS IN GENOMICS. GEMS is Genomics and Eco-evolution of Multi-scale Symbioses. Included in all this are short introductions to Illumina and Nanopore sequencing. I thought I would give these to you as blogs, this one on Illumina and another to come on Nanopore, and eventually one on PacBio. Note that we recently posted a blog on next generation sequencing, i.e. long read sequencing methods. That has a different flavor from what I’m doing here.
Tag: metagenomics
An up-date on next generation sequencing and scaffolding, 04/2021
Our last blog on third generation sequencing is still mostly relevant and current (here), so this post is an update where improvements are noted. The methods have matured a lot. The players are still largely the same: PacBio, Nanopore, Bionano and Hi-C. 10x has dropped out of the genomics field to focus almost exclusively on…… Read more »
Taxa annotation for shotgun sequenced metagenomic data
Tools to use for shotgun sequencing metagenomic data taxa annotation
Rarefaction curves for metagenomic datasets
Steps to plot rarefaction curves for WGS metagenomic datasets
Long read technologies potential to study the microbial world
Some interesting studies that are possible with the help of long-read technologies to study microbes.
Reconstructing genomes from metagenomes
The steps commonly taken to reconstruct genomes/metagenome-assembled genomes from shotgun sequenced metagenomes
Running Anvi’o on Jetstream
Anvi’o is now available as pre-configured VM on Jetstream
Third Generation Sequencing Technologies for Microbiome Analysis
Third generation sequencing technologies for microbiome/metagenomic studies.