NCGAS is teaching a course in genomics, called GEMS: WORKFLOWS IN GENOMICS. GEMS is Genomics and Eco-evolution of Multi-scale Symbioses. Included in all this are short introductions to Illumina and Nanopore sequencing. I thought I would give these to you as blogs, this one on Illumina and another to come on Nanopore, and eventually one on PacBio. Note that we recently posted a blog on next generation sequencing, i.e. long read sequencing methods. That has a different flavor from what I’m doing here.
A one-stop list of our most popular differential expression resources for background, tutorials, and code to do your own project.
Our last blog on third generation sequencing is still mostly relevant and current (here), so this post is an update where improvements are noted. The methods have matured a lot. The players are still largely the same: PacBio, Nanopore, Bionano and Hi-C. 10x has dropped out of the genomics field to focus almost exclusively on…… Read more »
We featured Dr. Petra Lenz in our first webinar, and share tools to get you started doing similar work.
Since our first transcriptomics workshop in 2018, many cutting-edge RNA sequencing technologies have become more popular. Some of these methods, like Iso-Seq, have made their way into non-medical fields. Direct and single-cell RNA sequencing methods, on the other hand, are used almost exclusively in cancer biology, biomarker discovery, and neurobiology.
Using KEGG tools online to make pathway figures!
Wondering about this last step in the pipeline and how it is filtering all those assemblies? Here’s the details!
The Broad’s RNA assembler Trinity is one of the most popular tools for assembling short reads (~100-150 base pairs) of RNA into the transcripts (~200-15000bp) produced by an organism. According to recent git pull stats, over 16,000 people have downloaded the program over 4.4 million times since 2015. And that number is increasing every…… Read more »