Why Nanopore Just out (March 1st 2021): New nanopore sequencing chemistry in developers’ hands; set to deliver Q20+ (99%+) “raw read” accuracy (modified enzyme, tweaked run conditions and further improved base calling model in the Bonito base caller) Nanopore reads are much longer than PacBio, they can reach 330 kbp in length, even exceeding 2Mb. Yield/cell is 245Gb. It can be used for both…… Read more »
Sequencing Methods
Introduction to Illumina Sequencing
NCGAS is teaching a course in genomics, called GEMS: WORKFLOWS IN GENOMICS. GEMS is Genomics and Eco-evolution of Multi-scale Symbioses. Included in all this are short introductions to Illumina and Nanopore sequencing. I thought I would give these to you as blogs, this one on Illumina and another to come on Nanopore, and eventually one on PacBio. Note that we recently posted a blog on next generation sequencing, i.e. long read sequencing methods. That has a different flavor from what I’m doing here.