Why Nanopore Just out (March 1st 2021): New nanopore sequencing chemistry in developers’ hands; set to deliver Q20+ (99%+) “raw read” accuracy (modified enzyme, tweaked run conditions and further improved base calling model in the Bonito base caller) Nanopore reads are much longer than PacBio, they can reach 330 kbp in length, even exceeding 2Mb. Yield/cell is 245Gb. It can be used for both…… Read more »
NCGAS is teaching a course in genomics, called GEMS: WORKFLOWS IN GENOMICS. GEMS is Genomics and Eco-evolution of Multi-scale Symbioses. Included in all this are short introductions to Illumina and Nanopore sequencing. I thought I would give these to you as blogs, this one on Illumina and another to come on Nanopore, and eventually one on PacBio. Note that we recently posted a blog on next generation sequencing, i.e. long read sequencing methods. That has a different flavor from what I’m doing here.
Our last blog on third generation sequencing is still mostly relevant and current (here), so this post is an update where improvements are noted. The methods have matured a lot. The players are still largely the same: PacBio, Nanopore, Bionano and Hi-C. 10x has dropped out of the genomics field to focus almost exclusively on…… Read more »
This blog is brought to you by NCGAS undergraduate intern Christina Campbell and is inspired by the curiosity of former NCGAS Co-PI Craig Stewart (who is now enjoying well-earned retirement). This is part of a multi-part series on exploring questions about epigenetics in beetles. Follow along as we determine which species have methylation enzymes, how these patterns are organized on the species tree of beetles, and how you can explore possible patterns of methylation – entirely with bioinformatics!
Available resources and tools through NCGAS in support for COVID19 research