On April 12, 2021, Carbonate is transitioning to the Slurm workload manager. We compiled the most essential information to work with SLURM, including information on converting your job script from Torque.
Since our first transcriptomics workshop in 2018, many cutting-edge RNA sequencing technologies have become more popular. Some of these methods, like Iso-Seq, have made their way into non-medical fields. Direct and single-cell RNA sequencing methods, on the other hand, are used almost exclusively in cancer biology, biomarker discovery, and neurobiology.
The NCGAS team finds that many users unfamiliar with SRA-Toolkit or the command line struggle with configuring the program or parsing its multiple commands and options. Here, I aim to condense and distill this information so that a beginner can use the SRA to their heart’s content!
Visualizing the distribution of scaffold size in an assembly is a nice complement to basic summary statistics. In this blog post, I’ll describe how to plot a histogram of scaffold lengths, using the new mOrnAna1 platypus assembly as an example.