We hosted Jean-Francois Gout of Mississippi State University this month. Jean-Francois did his post-doc work with the Lynch Lab at Indiana University and then Arizona State University (when the Lynch Lab relocated). He spoke about RNA transcription error and how to detect and study them using novel circle-sequencing of the RNA to produce multiple copies in a linear format (apparently a method borrowed from virology). Like PacBio HiFi, these replicates allow you to distinguish between sequencing error and real variation in the transcripts. Jean-Francois’s talk had a lot of really nice visuals – definitely worth a look!
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