Our new article explains, in detail, our protocols to study the lens epithelial monolayer! We discuss techniques for RNA isolation, protein extraction, protein preparation for capillary electrophoresis, flat mounting, and immunostaining. Traditional methods require pooling of many more epithelial sheets as opposed to our methods that can use as little as two epithelial peels. Read it here: Methodologies to unlock the molecular expression and cellular structure of ocular lens epithelial cells.
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